Some Factors Which Affect the Enzymatic Digestion of Ribonucleic Acid
نویسندگان
چکیده
منابع مشابه
[Some factors which affect the enzymatic digestion of ribonucleic acid].
The results of Klee and Richards (1) are in disagreement with the conclusions of Anfmsen et al. (2-4) that concentrated urea has no effect on the activity of bovine pancreatic ribonuclease. The possibility exists that differences in enzymatic activity might be also caused by alteration of the substrate rather than of the enzyme upon treatment with these agents. In examining the effect of condit...
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The discovery of enzymes which catalyze the deoxyribonucleic acid-dependent synthesis of ribopolynucleotides (l-6) stimulated an immediate inquiry into the role played by DNA in thisunique reaction. The speculation that DNA might serve as a template for the polymerization of ribonucleotides into a complementary ribonucleic acid molecule was confirmed by studies from a number of different groups...
متن کاملThe Enzymatic Methylation of Ribonucleic Acid and Deoxyribonucleic Acid. Vii. the Methylation of Ribonucleic Acid.
Preceding publications from this laboratory have shown that the methylation of soluble ribonucleic acid is catalyzed by at least six discrete enzymes (l-3) which have been purified from extracts of Escherichia coli W. Two of these enzyme preparations catalyze the formation of l-methylguanine residues in SRNA,1 while a third produces 7-methylguanine. The others catalyze the methylation of uracil...
متن کاملThe Enzymatic Phosphorylation of Ribonucleic Acid and Deoxyribonucleic Acid
The specificity of the enzyme purified from TZ-infected Escherichia coli which catalyzes the phosphorylation of 5’hydroxyl ends of ribonucleic acid and deoxyribonucleic acid has been examined. The enzyme also catalyzes the phosphorylation of 5’-hydroxyl groups of polynucleotides as well as 3’-mononucleotides. There is no detectable phosphorylation of nucleosides and adenosine 2’-phosphate is vi...
متن کاملThe Enzymatic Synthesis of Ribonucleic Acid. v. the Interaction of Ribonucleic Acid Polymerase with Nucleic Acids.
Numerous reports have been published on the properties and reaction mechanism of the highly purified enzyme preparations that catalyze the deoxyribonucleic acid-directed synthesis of ribonucleic acid (l-6). The various RNA polymerases tudied, although differing in some respects, show a remarkable similarity in their cofactor requirements, reaction kinetics, physical properties, and facility to ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1959
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)70040-9